trace element solution dsmz-medium 141 Search Results


dsmz medium 141  (DSMZ)
94
DSMZ dsmz medium 141
Dsmz Medium 141, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trace+element+solution+dsmz-medium+141/Methanogenium+sp/pm21304750-52-10-10
Average 94 stars, based on 1 article reviews
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m voltae salts  (DSMZ)
92
DSMZ m voltae salts
Fig. 1. A. Representation of the gene organization in the region of the smc gene in wild-type M. <t>voltae</t> and in M. voltae M6 smc::pac. The smc gene and two flanking predicted ORFs are shown. Both flanking ORFs are coded for on the other strand. The two probes for Southern blots (SMC-N and SMC-I) are shown as bold lines above the gene. EcoRI and NcoI restriction sites are also shown. Digestion with EcoRI and hybridization with SMC-I produces a single band of 3 kbp in the wild type and three bands of ª 500 bp, 1.4 kbp and 1.6 kbp as seen in the Southern blot in (B) (and also shown as black bars in A). Digestion with NcoI and hybridization with SMC-N produces a single band of kbp in the wild type and ª 5kbp in M6 as seen in the Southern blot in (B) (and also shown as black bars in A).
M Voltae Salts, supplied by DSMZ, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trace+element+solution+dsmz-medium+141/Methanococcus+voltae/pm15186409-234-53-56
Average 92 stars, based on 1 article reviews
m voltae salts - by Bioz Stars, 2026-09
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saline medium  (DSMZ)
93
DSMZ saline medium
Fig. 1. A. Representation of the gene organization in the region of the smc gene in wild-type M. <t>voltae</t> and in M. voltae M6 smc::pac. The smc gene and two flanking predicted ORFs are shown. Both flanking ORFs are coded for on the other strand. The two probes for Southern blots (SMC-N and SMC-I) are shown as bold lines above the gene. EcoRI and NcoI restriction sites are also shown. Digestion with EcoRI and hybridization with SMC-I produces a single band of 3 kbp in the wild type and three bands of ª 500 bp, 1.4 kbp and 1.6 kbp as seen in the Southern blot in (B) (and also shown as black bars in A). Digestion with NcoI and hybridization with SMC-N produces a single band of kbp in the wild type and ª 5kbp in M6 as seen in the Southern blot in (B) (and also shown as black bars in A).
Saline Medium, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trace+element+solution+dsmz-medium+141/SAL/pm30787924-44-0-3
Average 93 stars, based on 1 article reviews
saline medium - by Bioz Stars, 2026-09
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dsmz 141 medium  (DSMZ)
95
DSMZ dsmz 141 medium
Fig. 1. A. Representation of the gene organization in the region of the smc gene in wild-type M. <t>voltae</t> and in M. voltae M6 smc::pac. The smc gene and two flanking predicted ORFs are shown. Both flanking ORFs are coded for on the other strand. The two probes for Southern blots (SMC-N and SMC-I) are shown as bold lines above the gene. EcoRI and NcoI restriction sites are also shown. Digestion with EcoRI and hybridization with SMC-I produces a single band of 3 kbp in the wild type and three bands of ª 500 bp, 1.4 kbp and 1.6 kbp as seen in the Southern blot in (B) (and also shown as black bars in A). Digestion with NcoI and hybridization with SMC-N produces a single band of kbp in the wild type and ª 5kbp in M6 as seen in the Southern blot in (B) (and also shown as black bars in A).
Dsmz 141 Medium, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trace+element+solution+dsmz-medium+141/ML-1/pm15186409-219-13-13
Average 95 stars, based on 1 article reviews
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minimal medium  (DSMZ)
94
DSMZ minimal medium
Fig. 1. A. Representation of the gene organization in the region of the smc gene in wild-type M. <t>voltae</t> and in M. voltae M6 smc::pac. The smc gene and two flanking predicted ORFs are shown. Both flanking ORFs are coded for on the other strand. The two probes for Southern blots (SMC-N and SMC-I) are shown as bold lines above the gene. EcoRI and NcoI restriction sites are also shown. Digestion with EcoRI and hybridization with SMC-I produces a single band of 3 kbp in the wild type and three bands of ª 500 bp, 1.4 kbp and 1.6 kbp as seen in the Southern blot in (B) (and also shown as black bars in A). Digestion with NcoI and hybridization with SMC-N produces a single band of kbp in the wild type and ª 5kbp in M6 as seen in the Southern blot in (B) (and also shown as black bars in A).
Minimal Medium, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trace+element+solution+dsmz-medium+141/Bacillus+coagulans/10__1128_slash_aem__04054___15-38-12-42
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minimal medium - by Bioz Stars, 2026-09
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g10 minimal medium  (DSMZ)
93
DSMZ g10 minimal medium
Fig. 1. A. Representation of the gene organization in the region of the smc gene in wild-type M. <t>voltae</t> and in M. voltae M6 smc::pac. The smc gene and two flanking predicted ORFs are shown. Both flanking ORFs are coded for on the other strand. The two probes for Southern blots (SMC-N and SMC-I) are shown as bold lines above the gene. EcoRI and NcoI restriction sites are also shown. Digestion with EcoRI and hybridization with SMC-I produces a single band of 3 kbp in the wild type and three bands of ª 500 bp, 1.4 kbp and 1.6 kbp as seen in the Southern blot in (B) (and also shown as black bars in A). Digestion with NcoI and hybridization with SMC-N produces a single band of kbp in the wild type and ª 5kbp in M6 as seen in the Southern blot in (B) (and also shown as black bars in A).
G10 Minimal Medium, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trace+element+solution+dsmz-medium+141/Paenibacillus+polymyxa/pm22970819-133-12-7
Average 93 stars, based on 1 article reviews
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Image Search Results


Fig. 1. A. Representation of the gene organization in the region of the smc gene in wild-type M. voltae and in M. voltae M6 smc::pac. The smc gene and two flanking predicted ORFs are shown. Both flanking ORFs are coded for on the other strand. The two probes for Southern blots (SMC-N and SMC-I) are shown as bold lines above the gene. EcoRI and NcoI restriction sites are also shown. Digestion with EcoRI and hybridization with SMC-I produces a single band of 3 kbp in the wild type and three bands of ª 500 bp, 1.4 kbp and 1.6 kbp as seen in the Southern blot in (B) (and also shown as black bars in A). Digestion with NcoI and hybridization with SMC-N produces a single band of kbp in the wild type and ª 5kbp in M6 as seen in the Southern blot in (B) (and also shown as black bars in A).

Journal: Molecular microbiology

Article Title: Anucleate and titan cell phenotypes caused by insertional inactivation of the structural maintenance of chromosomes (smc) gene in the archaeon Methanococcus voltae.

doi: 10.1111/j.1365-2958.2004.04084.x

Figure Lengend Snippet: Fig. 1. A. Representation of the gene organization in the region of the smc gene in wild-type M. voltae and in M. voltae M6 smc::pac. The smc gene and two flanking predicted ORFs are shown. Both flanking ORFs are coded for on the other strand. The two probes for Southern blots (SMC-N and SMC-I) are shown as bold lines above the gene. EcoRI and NcoI restriction sites are also shown. Digestion with EcoRI and hybridization with SMC-I produces a single band of 3 kbp in the wild type and three bands of ª 500 bp, 1.4 kbp and 1.6 kbp as seen in the Southern blot in (B) (and also shown as black bars in A). Digestion with NcoI and hybridization with SMC-N produces a single band of kbp in the wild type and ª 5kbp in M6 as seen in the Southern blot in (B) (and also shown as black bars in A).

Article Snippet: To examine cell and nucleoid morphology, wild-type and mutant cells were grown in liquid culture to mid-exponential phase (OD600 a 0.4–0.6) and fixed by the addition of 25% glutaraldehyde to a final concentration of 3% (v/v) and incubated on ice for 20 min. As an alternative fixation, cells were harvested and resuspended in M. voltae salts (DSMZ medium 141 without yeast extract, peptone, vitamins), then fixed by the addition of 100% methanol at -20∞C.

Techniques: Hybridization, Southern Blot

Fig. 2. Micrographs of phase contrast (left column), DAPI epifluores- cence (middle column) and F420 autofluorescence (right column). A. Wild-type M. voltae. B. M6 cells. C. M6 cells showing the titan cell phenotype. Arrows point to anucleate cells. (A) was chosen to represent the wild- type because it contained the largest wild-type cell that we could find: most wild-type cells are much smaller. Bar = 2 mm.

Journal: Molecular microbiology

Article Title: Anucleate and titan cell phenotypes caused by insertional inactivation of the structural maintenance of chromosomes (smc) gene in the archaeon Methanococcus voltae.

doi: 10.1111/j.1365-2958.2004.04084.x

Figure Lengend Snippet: Fig. 2. Micrographs of phase contrast (left column), DAPI epifluores- cence (middle column) and F420 autofluorescence (right column). A. Wild-type M. voltae. B. M6 cells. C. M6 cells showing the titan cell phenotype. Arrows point to anucleate cells. (A) was chosen to represent the wild- type because it contained the largest wild-type cell that we could find: most wild-type cells are much smaller. Bar = 2 mm.

Article Snippet: To examine cell and nucleoid morphology, wild-type and mutant cells were grown in liquid culture to mid-exponential phase (OD600 a 0.4–0.6) and fixed by the addition of 25% glutaraldehyde to a final concentration of 3% (v/v) and incubated on ice for 20 min. As an alternative fixation, cells were harvested and resuspended in M. voltae salts (DSMZ medium 141 without yeast extract, peptone, vitamins), then fixed by the addition of 100% methanol at -20∞C.

Techniques: